Fig. 4. Effect of MEHA and MAHA hydrogels on RAW 264.7 in terms of: (a) and (b) cell proliferation after 1, 3 and 7 days of cell culture. Macrophage proliferation was performed by CCK8 assay using manufacturer's protocol; (c) and (d) interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-10 (IL-10) levels in basal condition. For (c) and (d), measurements were performed after 3 days of cell–material interaction and results (expressed as picograms per mL) are reported as mean ± standard deviation of 4 independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.0001; ns, no significant difference.