Fig. 6. Molecular simulation dynamics analysis of the vaccine construct with TLR-2. (A) Deformation plot of the vaccine-TLR2 complex and depiction of the minimal deformations, (B) atomic fluctuation analysis of the vaccine-TLR2 complex using B factors, (C) trajectory of binding of the vaccine candidate to TLR2 in the initial frames, with the atomic movement of the ligand towards TLR2, (D) the closest and stabilized conformation of the binding frame of the ligand to the TLR2 receptor through NMA, (E) covariance matrix analysis of the complex system, (F) stabilized cluster conformation of binding of the lead vaccine candidate to TLR2 in 1000 frames, (G) elastic network analysis, and (H) Eigen value assessment of the complex trajectory of the vaccine construct with the TLR-2 membrane receptor. (I) Variance model index of binding complex of vaccine constrtuct with TLR-2.