Fig. 3. Iron nanoparticles (IONPs) increase reactive oxygen species in immature bone marrow hematopoietic cells (lineage negative, Sca1 positive, c-kit negative, LSK⁻ cells). (a) Representative gating strategy for LSK⁻ BM populations and ROS level in different groups, measured by DCFDA using flow cytometry; (b) gating strategy for bone marrow LSK cells, LSK⁻ cell apoptosis in different treatment groups. Rats were treated with IONPs intravenously for 28 days in a 7 day interval.