Fitted parameters for tail-deuterated DMPC/DMPG membranes prior to and after exposure to 1 μM H–P–3W62 and D–P–3W62 peptide. The amount of peptide incorporated in the different layers is estimated based on the change in SLD observed after exposure to the peptide.
| Layer | d [Å] | Coverage [%] | SLD [10−6 Å−2] | Peptide [%] | d [Å] | Coverage [%] | SLD [10−6 Å−2] | Peptide [%] |
|---|---|---|---|---|---|---|---|---|
| Pristine SLB | ||||||||
| Water | 3 | 0 | — | — | 3 ± 1 | 0 | — | — |
| Head (inner) | 7 ± 1 | 82 ± 3 | 1.83 | — | 7 ± 1 | 84 ± 3 | 1.83 | — |
| Tail | 25 ± 1 | 94 ± 1 | 6.7 | — | 26 ± 1 | 98 ± 2 | 6.7 | — |
| Head (upper) | 7 ± 1 | 82 ± 3 | 1.83 | — | 7 ± 1 | 84 ± 3 | 1.83 | — |
| Total membrane thickness (Å) | 39 ± 2 | A mol = 62 ± 3 Å 2 | 40 ± 2 | A mol = 60 ± 3 Å 2 | ||||
| SLB after addition of | 1 μM H–P–3W62 | 1 μM D–P–3W62 | ||||||
| Water | 4 | 100 | — | — | 4 ± 1 | 0 | — | — |
| Head (inner) | 7 ± 1 | 82 ± 3 | 1.83 | — | 6 ± 1 | 84 ± 3 | 1.83 | — |
| Tail/peptide | 25 ± 1 | 92 ± 1 | 6.39 | 6 ± 1 | 26 ± 1 | 92 ± 2 | 6.37 | 6 ± 1 |
| Head/peptide | 7 ± 1 | 70 ± 4 | 1.56 | a | 7 ± 1 | 68 ± 3 | 1.95 | a |
| Total membrane thickness (Å) | 39 ± 2 | A mol N/A | 39s ± 2 | A mol N/A | ||||
| Peptide layer | 64 ± 3 | 6 ± 1 | 1.1/1.4/1.9 ± 0.3 | 64 ± 3 | 6 ± 1 | 4.3/4.6/5.2 ± 0.2 | ||
| PEG layer | 28 ± 4 | 2 ± 1 | 0.7 ± 0.3 | 28 ± 4 | 2 ± 1 | 7.2 ± 0.4 | ||
a
Cannot be determined with accuracy due to lack of contrast.