Fitted parameters for tail-deuterated DMPC/DMPG membranes prior to and after exposure to 10 μM 3W62, D–P–3W62 and D–P–3W62 peptide. The amount of peptide incorporated in the different layers is estimated based on the change in SLD observed after exposure to the peptide.
| Layer | d [Å] | Coverage [%] | SLD [10−6 Å−2] | Peptide [%] | d [Å] | Coverage [%] | SLD [10−6 Å−2] | Peptide [%] | d [Å] | Coverage [%] | SLD [10−6 Å−2] | Peptide [%] |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pristine SLB | ||||||||||||
| Water | 3 | 0 | — | — | 2 ± 1 | 0 | — | — | 3 | 0 | — | — |
| Head (inner) | 6 ± 1 | 87 ± 3 | 1.83 | — | 6 ± 1 | 80 ± 3 | 1.83 | — | 6 ± 1 | 82 ± 3 | 1.83 | — |
| Tail | 26 ± 1 | 96 ± 1 | 6.7 | — | 27 ± 1 | 92 ± 2 | 6.7 | — | 28 ± 1 | 96 ± 1 | 6.7 | — |
| Head (upper) | 6 ± 1 | 87 ± 3 | 1.83 | — | 6 ± 1 | 80 ± 3 | 1.83 | — | 6 ± 1 | 82 ± 3 | 1.83 | — |
| Total membrane thickness (Å) | 38 ± 2 | A mol = 61 ± 3 Å 2 | 39 ± 2 | A mol = 60 ± 3 Å 2 | 40 ± 2 | A mol = 60 ± 3 Å 2 | ||||||
| SLB after addition of | 10 μM 3W62 | 10 μM H–P–3W62 | 10 μM D–P–3W62 | |||||||||
| Water | 3 | 0 | — | — | 3 ± 1 | 0 | — | — | 3 | 0 | — | — |
| Head (inner) | 7 ± 1 | 82 ± 3 | 1.8 | 9 ± 4 | 6 ± 1 | 84 ± 3 | 1.83 | — | 6 ± 1 | 82 ± 3 | 1.81 | |
| Tail/peptide | 26 ± 1 | 88 ± 1 | 5.9 | 14 ± 1 | 26 ± 1 | 87 ± 2 | 6.38 | 5 ± 1 | 28 ± 1 | 90 ± 1 | 6.37 | 6 ± 1 |
| Head/peptide | 7 ± 1 | 79 ± 4 | 1.7 | 36 ± 2 | 7 ± 1 | 68 ± 3 | 1.74 | 7 ± 1 | 76 ± 4 | 2.3 | ||
| Total membrane thickness (Å) | 41 ± 2 | A mol N/A | 39 ± 2 | A mol N/A | 40 ± 2 | A mol N/A | ||||||
| First layer | 60 ± 1 | 34 ± 1 | 1.5/2.2/3.2 ± 0.2 | 8 ± 2 | 5 ± 1 | 0.7 ± 0.4 | 5 ± 3 | 5 ± 1 | 7 ± 0.5 | |||
| Second layer | 29 ± 4 | 14 ± 1 | 2.1 ± 0.3 | 29 ± 4 | 10 ± 1 | 2.1 ± 0.4 | ||||||
| Third layer | — | — | — | 27 ± 4 | 4 ± 1 | 0.7 ± 0.3 | 26 ± 4 | 2 ± 1 | 7 ± 0.5 | |||