Fig. 3. Optimization AptNCL/Lip surface density and cellular uptake. (A) Variable amounts of AptNCL post-inserted into fixed amount of Lip. (B) and (C) Mean Fluorescent Intensity (MFI) of rhodamine (LipRhod), obtained from flow cytometry analysis, vs. different AptNCL densities of AptNCL–Alexa–LipRhod of the formulation in MDA-MB-231 and MCF-7 cell lines. (D) and (E) Mean Fluorescent Intensity (MFI) of Alexa flour 647-tagged NCL (NCLAlexa) aptamer, obtained from flow cytometry analysis, vs. different AptNCL densities of the AptNCL-Alexa–LipRhod formulation in MDA-MB-231 and MCF-7 cell lines (mean ± SD, n = 3).
