Fig. 4. Flow cytometry analyses for the uptake of AptNCL-Alexa-LipRhod in cultured cells. (A) and (B) Flow cytometry histogram analysis and (C) and (D) the percent of positive cells after treatment with blank LipRhod, AptNCL-Alexa, and AptNCL-Alexa–LipRhod. (E) and (F) The mean fluorescent intensity (MFI) measurements of LipRhod and AptNCL-Alexa signals for the MDA-MB-231 and MCF-7 cell lines. Cells were treated with free AptNCL-Alexa (200 nM), or blank LipRhod (120 μM), or AptNCL-Alexa–LipRhod (200 nM AptNCL/120 μM LipRhod) and compared to untreated cells (mean ± SD, n = 3).
