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. 2022 May 1;163(2):495–506.e8. doi: 10.1053/j.gastro.2022.04.037

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Supplementary Figure 7 — Representative gating strategy. First cells were gated based on FSC and SSC; singlets were excluded by FSC-A vs FSC-H gate; live CD45⁺ high leukocytes were gated; live CD45+ high cells were separated in CD4⁺ and CD8⁺ cells. These cell populations were analyzed for intracellular IFN-γ, TNFα, IL-17, and GranzymeB. FSC, forward scatter; SSC, side scatter.