Enzyme immobilized on a nanofiber mat and enzyme activity.

Nanofibers	Immobilized enzymes	Type of immobilization method	Enzyme activity	Reference
PMMA/PANI	Laccase	Adsorption and covalent binding	Retained over 80% relative activity after 30 days of storage and after 10 consecutive catalytic cycles	117
PAN/gold salt	Laccase	Adsorption	The enzyme activity after 7 days was 62.7% of that on the first day	118
PS	α-Chymotrypsin	Covalent binding	The activity of the immobilized enzyme was 65% of that of the free enzyme, and showed high activity in both aqueous and organic media	122
PVA	Cellulase	Covalent binding	The activity of the immobilized enzyme was more than 65% of that of the free enzyme, and the initial activity remained 36% after being reused 6 times	124
PVA	Cyclodextrin glucanotransferase	Covalent binding	Compared with the ordinary film, the enzyme activity immobilized on the nanofiber increased by 31%	127
P(GMA-co-MA)-g-PEO	Lipase	Covalent binding	The maximum activity of 0.673 U mg−1	130
P(GMA-co-MA)/FP	Lipase	Covalent binding	The residual relative activity of the immobilized lipase was 62% after 7 reuses	131
PVA/BSA	Horseradish peroxidase	Covalent binding	The residual activity of the immobilized HRP were 73% after 11 reuse cycles	132
PVA/CS	β-Galactosidase	Add the enzyme directly to the solution	The catalytic activity of enzyme-NF was 57.03% of free enzyme activity at pH 6.8 and 4 °C	133
CNTs-COOH/GO	Lysozyme	Covalent binding	The enzyme was considerably active	136
PANCMA	Lipase	Covalent binding	The enzyme loading and the activity retention of the immobilized lipase on the nanofibrous membrane increase from 2.36 ± 0.06 to 21.2 ± 0.7 mg g−1 and from 33.9 to 37.6%, respectively	137
PS/PSMA	Bovine carbonic anhydrase	Covalent binding, precipitation, and cross-linking	The enzyme maintained 65.3% of its initial activity after being incubated in aqueous solution at room temperature under shaking at 200 rpm for 868 days	138