Fig. 2. Validation of the differential expressions of the glycopatterns in pooled saliva from HVs and T2DM patients. (A) The salivary proteins from HV and T2DM groups were separated by 10% SDS-PAGE and transferred onto a PVDF membrane. The 30 μg of Cy 5 labelled LEL, VVA and Jacalin were incubated with membranes, and the images were acquired using a STORM fluorImager respectively. The difference protein bands between HV and T2DM samples are marked with red frames. (B) The gray value of the difference protein bands was measured using the ImageJ software.
