Fig. 3. MALDI-TOF/TOF-MS spectra of the sialic acid-derivatized N-linked glycan from the LEL affinity glycoproteins of the saliva from HVs and T2DM patients. The salivary glycoproteins that contained LacNAc were isolated by LEL-magnetic particle conjugates, and derivatized N-glycans from HV (A) and T2DM samples (B) were released and characterized by MALDI-TOF/TOF-MS. Detailed glycan structures were annotated using the GlycoWorkbench software. Proposed structures and their m/z values were shown for each peak. The LacNAc-containing N-glycan peaks that increased or decreased in patients with T2DM are marked with red dotted lines or yellow dotted lines, respectively. (C) Number of N-glycan peaks (LacNAc-contained N-glycan peaks labeled in red) released from LEL-isolated glycoproteins from HV and T2DM samples. (D) Cross-correlation of the LacNAc-containing N-glycan peaks from HVs and T2DM patients are presented in the Venn diagram.