LacNAc-containing O-glycan peaks of salivary glycoproteins showed increased glycopatterns in patients with T2DM compared to HVs^a.

No	Calculated m/z	Ion type	Predicted components	Proposed N-glycan structureb	Relative intensities of O-glycans		Fold-changec (T2DM patients/HVs)
					HVsc (%)	T2DM patients (%)
1	725.186	H	Hex1HexNAc2S1		ND	0.931	—
2	1037.280	Na	Hex1HexNAc2(α2,3)NeuAc1S1		ND	0.510	—
3	1069.279	Na	Hex2HexNAc2S1dHex1		ND	0.644	—
4	1133.376	Na	Hex2HexNAc2(α2,3)NeuAc1		ND	0.734	—
5	1334.491	Na	Hex1HexNAc3(α2,6)NeuAc1dHex1		ND	2.329	—
6	1449.554	Na	Hex1HexNAc2(α2,6)NeuAc2dHex1		ND	2.059	—
7	1562.470	Na	Hex2HexNAc3(α2,3)NeuAc1S1dHex1		ND	0.612	—
8	868.296	Na	HexNAc3dHex1		6.422	13.063	2.034
9	884.291	Na	Hex1HexNAc3		64.770	100.000	1.544
10	981.196	H	Hex2HexNAc2S2		5.656	8.517	1.506
11	1008.367	H	Hex1HexNAc3dHex1		11.611	19.463	1.676
12	1051.28	Na	Hex1HexNAc2(α2,3)NeuAc1S1		50.187	78.874	1.572
13	1121.38	Na	Hex2HexNAc2dHex2		5.500	11.368	2.067
14	1166.451	H	Hex1HexNAc3(α2,6)NeuAc1		57.772	91.382	1.582
15	1211.369	Na	Hex1HexNAc2(α2,6)NeuAc1S1dHex1		5.500	9.609	1.747
16	1279.434	Na	Hex2HexNAc2(α2,3)NeuAc1dHex1		8.642	24.486	2.833
17	1309.459	Na	Hex3HexNAc2(α2,3)NeuAc1		6.621	11.573	1.748

^a .

^bProposed glycan structure, monosaccharides are represented according to MS-tools from the GlycoWorkbench software. In O-glycans, the reducing end A or B was residue of –CH₂COOH from serine or residue of –CH(CH₃)COOH from threonine.

^c—, the denominator of the fold-change is zero.