Fig. 4. Reaction rate comparison of PP3B and AP3B. (A) The concentrations of PP3B and AP3B in the treated samples were fixed at 2.5 mM and 20 μM, respectively. UV exposure time was between 1 and 30 min. (B) Quantification of reaction progress. The fluorescent intensities of the ssDNA and dsDNA bands were calculated to determine the percent of crosslinked DNA for each time point. 100% dsDNA indicates a complete reaction.
