Figure 3. Effect of 32-134D treatment on hypoxia-induced gene expression in Hepa1-6 cells.

(A–C) Cells were exposed to 20% O₂ and vehicle (white bars), 1% O₂ and vehicle (blue bars), or 1% O₂ and 32-134D (red bars) for 24 hours and mRNAs were quantified by RT-qPCR and normalized to white (mean ± SEM, n = 4). *P < 0.05 versus white; ^#P < 0.05 versus blue (ANOVA with Bonferroni’s post hoc test).