Figure 8. Trogocytosed CD8⁺ T cells acquire peptide-MHC complexes in a Tim-3–mediated fashion.

(A–C) Representative dot plots (left) and summary data (right) showing frequencies of OVA257-264 tet⁺ and PD-1⁺Tim-3⁺tet⁺ CD8⁺ TILs (A), peptide-MHC complex (pMHC)/OVA257-264 H-2Kb⁺ Tim-3⁺CD11c⁺ and Tim-3⁺CD14⁺ cells (B), or pMHC/OVA257-264-H-2Kb⁺ PD-1⁺Tim-3⁺ CD8⁺ TILs (C) in B16-OVA (day 21) treated with indicated mAbs. Results shown are from 1 experiment, representative of 2 independent experiments (n = 5). Data are representative of 3 independent experiments. P values were obtained by 1-way ANOVA followed by Tukey’s multiple-comparison test (A and C). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Data indicate mean ± SD.