Figure 3. Effects of pharmacological and/or genetic targeting of HIF-1α on E0771 tumor growth in immunodeficient or immunocompetent mice.
(A) Experimental design. Three sublines of E0771 were generated by lentiviral transduction: scrambled shRNA (sh-Scr), shRNA against Hif1a (sh-Hif1a), and sh-Pdl1. For each, 0.5 × 106 cells were orthotopically transplanted into NSG or C57BL/6 mice (day 0), which received vehicle or echinomycin (LEM, 0.25 mg/kg) starting on day 6. (B) Effects of Hif1a or Pdl1 knockdown on E0771 growth among immunodeficient or immunocompetent recipients. (C) Effects of vehicle or LEM on sh-Scr, sh-Hif1a, or sh-Pdl1 E0771 growth in immunocompetent recipients. (D) Effects of vehicle or LEM on sh-Scr, sh-Hif1a, or sh-Pdl1 E0771 growth in immunodeficient recipients. In the graphs, tumor volumes are plotted as the mean ± SEM for each group (n = 5/group), with significance determined by 2-way ANOVA, and the data shown are representative of 2 experiments. *P < 0.05; ***P < 0.001.