Figure 3. Offspring of SG-operated mice are born SGA.
(A) Distribution of the weights of P1 pups of mice that underwent sham or SG surgery. (B) Litter size of mice that underwent sham or SG surgery. (C) P1 weight corrected for litter size in offspring of sham- or SG-operated mice. (D) Average corrected weight per litter in mice that underwent sham or SG surgery. (E) Fraction of SGA pups (white) in offspring of sham or SG-operated mice. (F) Fractions of α, β, and δ cells in the pancreata of P1 mice. (G and H) Representative image and quantification of Ki67 positive cells in pancreata of P1 mice. Blue: sham pups; red: SG pups. *P < 0.05, **P < 0.01 by 2-tailed Student’s t test for B–D and H; and by χ2 test for E and F. In B, sham pups n = 16, SG pups n = 10; in A and C, sham pups n = 72, SG pups n = 107; in D, sham pups n = 7, SG pups n = 10; in E, sham pups n = 26, SG pups n = 19. At least 8 islets/P1 pancreas were analyzed, and at least 1 pup per litter was analyzed in F and H.