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. 2001 Jun;45(6):1930–1933. doi: 10.1128/AAC.45.6.1930-1933.2001

FIG. 1.

FIG. 1

FIG. 1

Effect of azithromycin on growth and elastase and rhamnolipid production. (A) Bacterial strains were grown in Luria-Bertani (LB) medium in the absence (squares) or in the presence (circles, 2 μg/ml; upside triangles, 3 μg/ml; downside triangles, 4 μg/ml; diamonds, 5 μg/ml) of azithromycin. Growth curve determinations were repeated five times and the graph shows results from one typical experiment. (B) Supernatants of cells, grown either in the absence (squares) or the presence (circles) of 2 μg of azithromycin/ml, were collected at regular intervals and elastase activity was determined by the elastin Congo red assay. (C) PAO1(pECP60) (rhlA′-lacZ) was grown in LB medium either in the absence (squares) or the presence (circles) of 2 μg of azithromycin/ml, and β-Gal activities were assayed at regular time intervals. Inserted graphs in panels B and C show the corresponding growth curves. Results are the mean ± SD of three independent experiments performed in duplicate.