Filtered list of metabolites detected by 1D 1H NMR spectra. Metabolites detected by S-plot and VIP plot analysis (see Fig. S1 ESI) of 1D 1H NMR spectra of urine from rats treated by either 62.5 mg kg−1 or 125 mg kg−1 tetracycline. Refer to method section for Experimental details. Values differing significantly according to S-plot and VIP plot are shown. Doublet (d), triplet (t) and multiplet (m).
| Tetracycline treated samples | |||||||
|---|---|---|---|---|---|---|---|
| Metabolites | Chemical shift (ppm) | Multiplicities | P-Value | 62.5 mg kg−1 | 125 mg kg−1 | ||
| Fold change | VIP | Fold change | VIP | ||||
| Hippurate | 7.84 | d | 0.016 | 0.904 | — | 3.209 | 2.275 |
| Citrate | 2.54 | d | 0.011 | 0.926 | — | 1.480 | 2.279 |
| Lysine | 1.98 | m | 0.016 | 0.924 | 1.377 | 0.868 | 1.206 |