Amine donor screening in transamination of 1-phenylpropan-2-one (7a) with three different immobilised transaminasesa.
| Entry | Enzyme | Amine donor | Conversion [%] | ee [%] |
|---|---|---|---|---|
| 1 | ArR-TA | Isopropylamine | 27.8 | >99 (R) |
| 2 | sec-Butylamine | 83.0 | >99 (R) | |
| 3 | Allylamine | 8.7 | >99 (R) | |
| 4 | Benzylamine | 3.1 | >99 (R) | |
| 5 | ArRm-TA | Isopropylamine | 25.4 | 62.2 (R) |
| 6 | sec-Butylamine | 51.6 | 82.4 (R) | |
| 7 | Allylamine | 14.4 | 91.5 (R) | |
| 8 | Benzylamine | 8.0 | 82.4 (R) | |
| 9 | AtR-TA | Isopropylamine | 8.5 | >99 (R) |
| 10 | sec-Butylamine | 68.5 | >99 (R) | |
| 11 | Allylamine | 0.8 | >99 (R) | |
| 12 | Benzylamine | 2.8 | >99 (R) |
a
Reaction conditions: immobilised whole-cell TA biocatalyst (20 mg), 7a (10 mM), amine donor (100 mM), PLP (1 mM), sodium phosphate buffer (100 mM, pH 7.5), DMSO (5 v/v%), 30 °C, 24 h.