FIG. 6.

Medium stiffness hydrogels support increased number of muscle fibers. (A) Quantification of the number of muscle fibers in the zone of regeneration per muscle section revealed that medium stiffness hydrogels supported increased myogenesis. (B) Median minimum Feret diameter (dashed lines) was significantly reduced in NR treated muscle compared to native muscle (dotted lines, first and third quartiles). (C) Minimum Feret diameter frequency distribution curve shows a leftward shift toward smaller muscle fibers compared to uninjured muscle regardless of treatment type. Statistically significant differences from native (p < 0.05) are denoted by n (NR), l (low), and h (high). Data presented as mean ± SD (A, C). *p < 0.05. n = 3 muscles per experimental group. Color images are available online.