Skip to main content
. 2022 Apr 13;28(7-8):330–340. doi: 10.1089/ten.tea.2021.0117

FIG. 5.

FIG. 5.

Western blot analysis of CD133+ versus CD133− GBM6 cell specimens in response to matrix-bound HA or extracellular hypoxia. (A) CHCHD2 expression in CD133+ cells was significantly increased in GelMA hydrogels under hypoxia (vs. GelMA normoxia; GelMA+HAMA hypoxia). CHCHD2 expression in CD133− cells was significantly increased in HA containing hydrogels in response to extracellular hypoxia. (B) STAT3 expression was largely similar in both CD133+ and CD133− GBM6, with significant decreases in STAT3 phosphorylation in response to extracellular hypoxia, regardless of the presence or absence of matrix-bound HA. (C) Both CD133+ and CD133− GBM6 cells showed decreased expression of CD44 with addition of matrix-bound HA and in the presence of hypoxia; CD133+ GBM6 cells also showed decreased expression of CD44 under normoxia in response to matrix-bound HA. ^p < 0.05 for +/− hypoxia; *p < 0.05 for +/− HAMA. Color images are available online.