SPPS evaluation of three solvent mixtures on Wang PS and Rink Amide ChemMatrix® resin as reported by Ferrazzano et al.^41a.

Entry	Peptide	Solvent	Resin	Purity (%)
1	Aib-enkephalin	DMF	Wang PS	13.5
2	Aib-enkephalin	DMF	Rink Amide ChemMatrix®	53.0
3	Aib-enkephalin	Sulfolane/DEC (3 : 7)	Wang PS	72.8, 69.1b
4	Aib-enkephalin	Sulfolane/DEC (3 : 7)	Rink Amide ChemMatrix®	62
5	Aib-enkephalin	Cyrene™/DEC (3 : 7)	Wang PS	37.3, 72.0b
6	Aib-enkephalin	Anisole/DMC (7 : 3)	Rink Amide ChemMatrix®	72.1
7	Aib-ACP	DMF	Rink Amide ChemMatrix®	37.8
8	Aib-ACP	Sulfolane/DEC (3 : 7)	Rink Amide ChemMatrix®	10.0
9	Aib-ACP	Anisole/DMC (7 : 3)	Rink Amide ChemMatrix®	31.0
10	Octreotide	DMF	H-Thr(tBu)-ol-2CT-PS	88
11	Octreotide	Anisole/DMC (7 : 3)	H-Thr(tBu)-ol-2CT-PS	64.6, 82.3c, 84.9d

^aCoupling was performed for 1 h at a 0.2 M concentration using 3 equiv. of Fmoc-protected amino acid, 3 equiv. of DIC and 3 equiv. of Oxyma Pure.

^bDouble coupling 2 × 1 h of the Fmoc-Leu-OH amino acid.

^cCoupling of Fmoc-Cys-OH at 40 °C.

^dCoupling for all steps at 40 °C.