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. 2022 Apr 18;9:864188. doi: 10.3389/fcvm.2022.864188

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Copyright © 2022 Cui, Ye, Wang, Yang, Zhu, Hu, Lan, Huang, Wang, Gu, Yan, Ma, Qi and Luo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isolation and characterization of MCELNs. (A) Flow sheet on the isolation of MCELNs from Momordica. charantia (MC) using gradient density centrifugation. (B) Transmission electron microscope image of MCELNs, scale bar: 2 μm. (C) NTA analysis exhibited the diameter of MCELNs was around 106.0 nm. (D) Western blot analysis of EVs biomarker, including TSG101, CD63, and CD9 in MC supernatant and MCELNs. (E) Representative images on the uptake of PKH67-labled MCELNs (green) in H9C2 cells when co-cultured at 0, 6, 12, 24 h. The nucleus were stained with DAPI (blue), scale bar: 10 μm.