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. 2022 Apr 18;9:864188. doi: 10.3389/fcvm.2022.864188

Figure 2.

Figure 2

MCELNs enhanced the proliferation of H9C2 cells after radiation exposure. (A) H9C2 cells were previously treated with different doses of MCELNs (0, 0.5, 5, 10, 25 μg/mL) and then exposed to 16 Gy X-ray. After 48 h of culture, the cell viability of H9C2 cells was determined using a MTT assay. (B) Representative growth images of H9C2 cells after 48 h of culture with indicated treatment, scale bar: 200 μm. (C) Immunofluorescence staining (left) and quantitation (right) of Ki-67 (red) positive stained H9C2 cells after 48 h of culture with indicated treatment. The nucleus were stained with DAPI (blue), scale bar: 10 μm. Western blot analysis and quantitation on the expressions of PCNA (D), Cyclin D1 (E), and Cyclin B1 (F) in H9C2 cells after 48 h of culture with indicated treatment. IR (–/+): 0/16 Gy X-ray; MCELNs (–/+): 0/10 μg/mL. All data were represented as means ± SD (n = 3 independent experiments). The statistical significance was evaluated by one-way ANOVA followed by the Turkey's multiple comparisons test among groups.