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. 2022 Apr 18;9:870244. doi: 10.3389/fmed.2022.870244

Figure 3.

Figure 3

Global DNA methylation percentages of the TNFSF11 promoter CpG-rich region in women (I) and men (II) groups using Standard non-linear Models. Curve fitting plots for serial DNA methylation percentages of converted Human DNA controls at 0, 25, 50, 75, and 100%, red and black shapes respectively, with (A) methylated TNFSF11 primers and (B) unmethylated TNFSF11 primers. The curve fitting plot (A) shows a proportionate increasing percentage of the methylated DNA with a decreasing of the Cq values for the TNFSF11 methylated primers' reactions. The blue (A.I) and green (A.II) shapes illustrate resulted in DNA methylation percentages among case and control samples of quantitative amplifications with TNFSF11 methylated primers in women and men, respectively. The curve-fitting plot (B) shows a proportionate increasing percentage of the unmethylated DNA with decreasing of the Cq values for the TNFSF11 unmethylated primers' reactions. The pink (B.I) and purple (B.II) shapes illustrate resulted in DNA unmethylation percentages among case and control samples of quantitative amplifications with TNFSF11 unmethylated primers in women and men, respectively. The x-axis details the average of Cq values reported in each reaction. The right y-axis represents the average percent DNA methylation across the TNFSF11 promoter CpG-rich region. The left y-axis represents the average percent DNA unmethylation across the TNFSF11 promoter CpG-rich region.