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. Author manuscript; available in PMC: 2023 Apr 28.
Published in final edited form as: Cell. 2022 Apr 5;185(9):1506–1520.e17. doi: 10.1016/j.cell.2022.03.017

Figure 1. gli1 is required in male worms for female sexual development.

Figure 1.

(A) DAPI (grey) and Fast Blue BB (red) labeling of sexually immature adult “virgin” and sexually mature “paired” adult females. Fast Blue BB labels mature vitellocytes. (B) Schematic of the RNAi screening regime. (C) Number of eggs laid per day per female parasite between D12–14 after pairing following RNAi. n > 23 worm pairs for each treatment group, n = 3 biological replicates. (D, E) Evaluation of sexual development (center) or egg production (right) following RNAi of gli1 in (D) female worms or (E) male worms, followed by pairing with non-treated worms of the opposite sex. Fast Blue BB (red) and DAPI (grey) staining showing the vitellaria (left) and ovary (right) from female parasites on day 14 after pairing. n > 21 parasites for each treatment, n = 3 biological replicates. Egg production rates were examined with n > 23 worms for each treatment group. (F) Sagittal confocal section of FISH showing gli1 (magenta) mRNA enriched in the ventral part of a male worm (left) where the male-female interaction occurs (right). n > 10 parasites across 3 experiments. ** p < 0.01; *** p < 0.001; ns p > 0.05. Parametric t test. Error bars represent SD. Each data point represents an individual experiment. Numbers in corner indicate fraction of worms that similar to those presented/total number of worms examined. Scale bars, A, 500 μm; D, E, F, 100 μm.