KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| For WISH: Anti-Digoxigenin-AP | Sigma-Aldrich | Cat# 11093274910 RRID:AB_2734716 |
| For FISH: Anti-Digoxigenin-horseradish peroxidase | Roche | Cat# 11207733910 RRID:AB_514500 |
| For FISH: Anti-Fluorescein-horseradish peroxidase | Sigma-Aldrich | Cat# 11426346910 |
| For FISH: Anti-DNP-horseradish peroxidase | Vector Laboratories | Cat# MB-0603-5 |
| Bacterial and virus strains | ||
| MAX Efficiency™ DH10Bac™ Competent Cells for bacto-bac cloning | Thermo Fisher Scientific | Cat# 10361012 |
| Biological samples | ||
| N/A | ||
| Chemicals, peptides, and recombinant proteins | ||
| ABC169 media supplement: bovine washed red blood cells | Lampire Biological Products | Cat# 7240808 |
| ABC169 media supplement: porcine cholesterol concentrate | Rocky Mountain Biologicals | Cat# PCC-BDG-VCG |
| ABC169 media supplement: L-ascorbic acid | Sigma-Aldrich | Cat# A5960 |
| tricaine | Sigma-Aldrich | Cat# A5040 |
| Fast Blue BB | Sigma-Aldrich | Cat# F3378 |
| EdU (5-ethynyl-2’-deoxyuridine) | Invitrogen | Cat# E10187 |
| DAPI | Sigma-Aldrich | Cat# D9542 |
| FAST SYBR Green Master Mix | Bio-rad | Cat# 1725122 |
| cOmplete protease inhibitor | Sigma-Aldrich | Cat# 11873580001 |
| Ni-NTA agarose | Qiagen | Cat# 4561 |
| Strep-Tactin Sepharose resin | Fisher Scientific | Cat# NC9891255 |
| [32P]PPi | PerkinElmer | Cat# NEX019001MC |
| [3H]βAlanine | American Radiolabeled Chemicals | Cat# ART 0205 |
| [13C3]βAlanine | Cambridge Isotope Laboratories | Cat# clm-8755-PK |
| βAlanine | Sigma-Aldrich | Cat# 146064 |
| mcBATT | Synthesized by MuseChem, owned by this paper | Batch# M21X01198 |
| swBATT | This paper | Order# MCC-RC-106 |
| Critical commercial assays | ||
| Direct-zol RNA MiniPrep | Genesee Scientific | Cat# 11-330 |
| Illumina TruSeq stranded mRNA library kit | Illumina | Cat# 20020595 |
| iScript cDNA Synthesis Kit | Bio-Rad | Cat# 1708891 |
| Deposited data | ||
| gli1 RNAi RNAseq | GEO | GSE184849 |
| BATT treatment female RNAseq | GEO | GSE191062 |
| Single sex female RNAseq 1 | WormBase ParaSite | ERR1328130_1, ERR1328130_2 |
| Single sex female RNAseq 2 | WormBase ParaSite | ERR1328191_1, ERR1328191_2 |
| Single sex female RNAseq 3 | WormBase ParaSite | ERR1328215_1, ERR1328215_2 |
| Mixed sex female RNAseq 1 | WormBase ParaSite | ERR1328194_1, ERR1328194_2 |
| Mixed sex female RNAseq 2 | WormBase ParaSite | ERR1328218_1, ERR1328218_2 |
| Mixed sex female RNAseq 3 | WormBase ParaSite | ERR1328156_1, ERR1328156_2 |
| Experimental models: Cell lines | ||
| Sf9 | ATCC | CRL-1711 |
| Experimental models: Organisms/strains | ||
| Parasites: Schistosoma mansoni | NIAID Schistosomiasis Resource Center of the Biomedical Research Institute | NMRI |
| Infected snails: Biomphalaria glabrata | NIAID Schistosomiasis Resource Center of the Biomedical Research Institute | NMRI |
| Infected female mice | NIAID Schistosomiasis Resource Center of the Biomedical Research Institute | Swiss-Webster |
| Oligonucleotides | ||
| Forward and reverse primers for riboprobe and dsRNA synthesis | This paper, Table S5 | N/A |
| Forward and reverse primers for qPCR detection | This paper, Table S5 | N/A |
| Recombinant DNA | ||
| pJC53.2-gli1 for RNAi and in situ hybridization | This paper | Plasmid# pJW186, Smp_266960 |
| pJC53.2-Sm-nrps for RNAi and in situ hybridization | This paper | Plasmid# pRC90, Smp_158480 |
| pJC53.2-7b2 for in situ hybridization | This paper | Plasmid# pLZ180, Smp_073270 |
| pJC53.2-tppp2 for in situ hybridization | This paper | Plasmid# pLZ266, Smp_097490 |
| pJC53.2-calp for in situ hybridization | This paper | Plasmid# pGW17, Smp_214190 |
| pJC53.2-tpm2 for in situ hybridization | This paper | Plasmid# pLZ93, Smp_031770 |
| pJC53.2-p48 for in situ hybridization | This paper | Plasmid# pLZ31, Smp_241610 |
| pJC53.2-bmpg for in situ hybridization | This paper | Plasmid# pLZ262, Smp_078720 |
| Protein expression construct: pFastBac1-His6-StrepII-TEV-SmNRPS, codon optimized to insect cells | This paper | Plasmid# pRC135_OP |
| Protein expression construct: pFastBac1-His6-StrepII-TEV-SmNRPS_S892A, codon optimized to insect cells | This paper | Plasmid# pRC135.S892A_OP |
| Protein expression construct: pFastBac1-His6-StrepII-TEV-Ebony | This paper | Plasmid# pRC136, protein NCBI: NM_079707.4 |
| Software and algorithms | ||
| MassHunter Profinder software | Agilent Technologies | Version B.08.00 SP3 |
| T-Coffee sequence alignment | Snapgene 4.3.4 | http://tcoffee.crg.cat/ |
| MUSCLE sequence alignment | Snapgene 4.3.4 | https://www.ebi.ac.uk/Tools/msa/muscle/ |
| STAR | Dobin et al., 2013 [1] | N/A |
| DESseq2 | Love et al., 2014 [2] | N/A |
| Other | ||
| 40 μm cell strainer for female/male worm separation | Thermo Fisher Scientific | Cat# 22-363-547 |
| speed vacuum | Thermo Fisher Scientific | Cat# SPD1010-115 |
| 6550 iFunnel Q-TOF mass spectrometer | Agilent Technologies | Cat# G6550BA |
| Acquity UPLC® HSS T3 column | Waters | Cat# 186003539 |
| AB Sciex 6500+ QTRAP® mass spectrometer | Framingham | N/A |
| Nexera X2 UHPLC/HPLC | Shimadzu and Columbia | N/A |
1.
Dobin, A., et al., STAR: ultrafast universal RNA-seq aligner. Bioinformatics, 2013. 29(1): p. 15–21.
2.
Love, M.I., W. Huber, and S. Anders, Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol, 2014. 15(12): p. 550.