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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Dev Biol. 2022 Mar 25;486:56–70. doi: 10.1016/j.ydbio.2022.03.009

Figure 5. Microtubules in minus-end-out, but not plus-end-out, regions of mosaic arbors show the dendritic response to neurite severing.

Figure 5.

A. Schematics showing the dendrite injury assay for control neurons. Lightning bolt indicates site of severing.

B. Quantification of EB1-GFP dynamics before and after dendrite injury in control neurons, n = 10 animals.

C. Schematics showing axon injury assay in control neurons.

D. Quantification of EB1-dynamics before and after axon injury in control neurons, n = 10 animals. P<0.05 when the outcomes (increase in comet number vs no increase) were compared using the Fisher’s Exact test between 5B and 5D.

E. Schematics showing the assay to measure EB1-GFP dynamics after injury in mosaic ddaC neurons. Plus-end-out neurites are marked with red lines and minus-end-out with blue lines.

F. Quantification of EB1-GFP dynamics before and after injury in mosaic neurons (n = 16 animals). P<0.05 when the outcomes (increase in comet number vs no increase) were compared using the Fisher’s Exact test between minus-end-out and plus-end-out neurites.