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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Dev Biol. 2022 Mar 25;486:56–70. doi: 10.1016/j.ydbio.2022.03.009

Figure 7. Plus-end-out neurites in mosaic arbors have reduced branching.

Figure 7.

A. Representative images of control and mosaic ddaC neurons expressing the mCD8-GFP to visualize morphology. Secondary branches (quantified in B) are marked with purple dashed lines. Terminal branches (quantified in C) are marked with orange lines. In mosaic neurons microtubule polarity of neurites is marked with blue dashed lines for minus-end-out and red dashed lines for plus-end-out.

B. Quantification of the length of secondary branches in plus-end-out and minus-end-out neurites of mosaic neurons. ns, P>0.05 when analyzed with student’s t-test (n=12 animals).

C. Quantification of the number of terminal branches (normalized to length) in plus-end-out and minus-end-out neurites in mosaic arbors. **, P<0.01 when analyzed with student’s t-test (n=12 animals).

D. Representative kymographs from time-lapse videos of neurites in mosaic arbors expressing EB1-TagRFP-T. Blue lines mark the minus-end-out microtubules while red lines mark the plus-end-out microtubules. Scale bar: x axis = 10 µm, y axis = 60 seconds.