Skip to main content
. 2022 Apr 29;5(9):e202101337. doi: 10.26508/lsa.202101337

Figure 5. In poorly radioimmunogenic tumors, exogenous adjuvant increases the number of tumor-migratory conventional dendritic cells in the TdLN.

Figure 5.

(A) Experiment design: Panc02-SIY (P2SIY) tumors were established in Kaede mice. When tumors reached 5–6 mm average diameter, they were photoconverted with violet light and treated with 12-Gy radiation in combination 50 μg intratumoral poly I:C. (B) Gating on converted (Kaede-red+) DCs (CD11c+ MHC-II+) and the frequency of mig CD103+ versus mig CD11b+ conventional dendritic cells were assessed. (C) The TdLN was harvested 1 d after treatment to assess (i) the total number of converted mig CD103+ DCs, (ii) frequency of converted mig CD103+ DCs expressing CD80, and (iii) the median fluorescence intensity (MFI) on CD103+ DCs. (D) The TdLN was harvested 1 d after treatment to assess (i) the total number of converted mig CD11b+ DCs, (ii) frequency of converted mig CD11b+ DCs expressing CD80, and (iii) the median fluorescence intensity (MFI) on CD11b+ DCs. Data represent the mean ± SEM of each group. Results shown are representative of two independent experiments with n = 6 animals/group. *P < 0.05, ***P < 0.001 and ****P < 0.0001.