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Fig. 8. (A) Cell viability assay: histogram showing the mean absorbance value obtained from the MTT assay in A549 cells after treatment with different concentration (0 μM to 100 μM) for 3.5 h. The error bars indicate the standard error calculated from two independent experiments in duplicate. (B) Phase-contrast images of morphological changes observed in A549 cells after exposure of different concentrations of C16C4C16Br2 surfactant for 3.5 h.