Grading system of the macroscopic signs in the in vivo tolerance studies of insulin loaded chitosan nanoparticles^a.

Grade	Time
	Control			Plain insulin solution			C4T4I4
Time	0.5 h	4.0 h	16.0 h	0.5 h	4.0 h	16.0 h	0.5 h	4.0 h	16.0 h
Discomfort	0	0	0	0	0	0	0	0	0
Discharge	0	0	0	0	1	0	1	0	0
Cornea	0	0	0	0	0	0	0	0	0
Conjunctiva	0	0	0	0	0	0	0	0	1
Lids	0	0	0	0	0	0	0	0	0

^a n = 3.