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. 2022 Apr 11;28(2):91–103. doi: 10.1177/17534259221090679

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Validation of PMA-induced differentiation of THP-1 monocytes into M0-Mφs. (A) Representative phase contrast inverted microscopy images of THP-1 monocytes and M0-Mφs under × 400 magnification (scale bar: 50 μm). (B) Representative flow cytometry dot plots showing the forward-scatter (FSC) and side-scatter (SSC) properties of THP-1 monocytes and M0-Mφs. (C) Representative flow cytometry histogram plots demonstrating the surface expression of CD11b and CD11c on THP-1 monocytes and M0-Mφs. (D) Column bars representing mean values of the percentage (%) and geometric mean fluorescence intensity (MFI) of CD11b⁺ and CD11c⁺ cells from 3 independent experiments (with each condition performed in duplicate). Error bars represent SEM. Statistically significant differences were determined by unpaired t-test **P < 0.01; ***P < 0.001; ****P < 0.0001.