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Modulation of radiation-induced cell cycle arrest by SOCS1, reducing IR-induced G2/M arrest and promoting G1 arrest. Cell cycle analysis of HCT116/p53 wt cells stably transfected with HA and HA-SOCS1 or sh and shSOCS1 were performed at 24 h post irradiation of 5 Gy by flow cytometry using the Cellquest program as described in the text (A, B). Cells were subjected to immunoblotting to analyze expression levels of cell cycle marker proteins (C, D: Supplementary Fig. 6A).
