Fig. 4. Evaluation of zone of inhibition of Md-Lec–pCuS NPs against (A) A. hydrophila, (B) E. fecalis and (C) S. iniae. L – 50 μg ml⁻¹ Md-Lec, N – 3.13 μm pCuS NPs, NL – Md-Lec–pCuS NPs, which is composed of 50 μg ml⁻¹ Md-Lec and 3.13 μm pCuS NPs. It is noted the larger zone was observed in NL, suggesting that the antibacterial activity of Md-Lec is enhanced when combined with pCuS nanoparticles.