Central composite design (CCD) matrix of the four parameters with the experimental values from photo-induced O-AgNP biosynthesis (light intensity: 75.90 μmol m⁻² s⁻¹).

Std. order	Initial pH level (X1)	AgNO3 concentration (mM) (X2)	O. limnetica extract concentration (mL) (X3)	Incubation period (h) (X4)	Intensity (nm)
1	5.7	0.2	2.25	16.5	−0.0016
2	7.7	0.2	2.25	16.5	0.0060
3	5.7	0.4	2.25	16.5	−0.0022
4	7.7	0.4	2.25	16.5	0.0391
5	5.7	0.2	4.75	16.5	−0.0221
6	7.7	0.2	4.75	16.5	0.0043
7	5.7	0.4	4.75	16.5	−0.0516
8	7.7	0.4	4.75	16.5	0.3450
9	5.7	0.2	2.25	37.5	0.0326
10	7.7	0.2	2.25	37.5	0.3054
11	5.7	0.4	2.25	37.5	0.2855
12	7.7	0.4	2.25	37.5	0.3064
13	5.7	0.2	4.75	37.5	0.3220
14	7.7	0.2	4.75	37.5	0.4584
15	5.7	0.4	4.75	37.5	0.3866
16	7.7	0.4	4.75	37.5	0.6753
17	4.7	0.3	3.50	27.0	−0.0029
18	8.7	0.3	3.50	27.0	0.1670
19	6.7	0.1	3.50	27.0	0.0427
20	6.7	0.5	3.50	27.0	0.1479
21	6.7	0.3	1.00	27.0	0.1133
22	6.7	0.3	6.00	27.0	0.2255
23	6.7	0.3	3.50	6.0	0.2979
24	6.7	0.3	3.50	48.0	0.8695
25	6.7	0.3	3.50	27.0	0.4324
26	6.7	0.3	3.50	27.0	0.4324
27	6.7	0.3	3.50	27.0	0.4309
28	6.7	0.3	3.50	27.0	0.4319
29	6.7	0.3	3.50	27.0	0.4331
30	6.7	0.3	3.50	27.0	0.4339
31	6.7	0.3	3.50	27.0	0.4324