Figure 5. Teneurin4^WT adopts the compact dimer conformation in the presence of calcium.

1. Dimensionless Kratky plots for Teneurin4^WT (left) and Teneurin4^Mut (right) in physiological calcium concentrations (+ Ca²⁺; black), low calcium concentrations (+/− Ca²⁺; blue and red, respectively) and no calcium (− Ca²⁺; grey). I(0) and Rg were obtained from Guinier analysis (Fig EV5A and B). Crosshairs indicate the maximum position for a fully folded globular protein.
2. Pair‐distance distribution plots for Teneurin4 proteins colored as in A.
3. Full‐length Teneurin4^WT ECD model (inset) and fit (red line) against the Teneurin4^WT data in physiological calcium concentrations (black dots).
4. Distributions of radii of gyration of ensembles of structures selected from the random pool (dotted line), to best represent the SAXS data of each of the Teneurin4 variants, colored as in A.