TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Prototroph | |
| KG2225 | mexA::res-ΩSm of PAO1; Smr | This study |
| KG2504 | ampC::ΩSm of PAO1; Smr | This study |
| KG2505 | mexA::res-ΩSm of KG2504; Smr | This study |
| OCR1 | MexAB-OprM-overproducing nalB mutant of PAO1 | 24 |
| Plasmids | ||
| pTAK1900 | E. coli-P. aeruginosa shuttle cloning vector pAK1900 (38) carrying tet from pBR322 in bla; Tcr | This study |
| pKMF010 | pTAK1900 carrying the 3.5-kb SphI-PvuI fragment encompassing lacIq-Ptrc-ompF-rrnBT1T2; Tcr, inducible OmpF production | This study |
| pKMF012 | ompF-deficient pKMF010; Tcr | This study |
| pKMF014 | pTAK1900 carrying the 3.5-kb SphI-PvuI fragment encompassing lacIq-Ptrc-ompF-rrnBT1T2 and the 4.1-kb EcoRI-PstI fragment encompassing the IMP-1 gene; Tcr, inducible OmpF production and constitutive IMP-1 production | This study |
| pKMF016 | ompF-deficient pKMF014; Tcr, constitutive imipenemase production | This study |
a
Abbreviations: Smr, streptomycin resistant; Tcr, tetracycline resistant; rrnBT1T2, transcription terminator sequences; IMP-1, carbapenemase.