TABLE 2.
Strain/plasmid | Phenotypeb
|
Permeability ratec (%) of:
|
|||
---|---|---|---|---|---|
MexAB | OmpF | Imipenem | Faropenem | Sulopenem | |
PAO1/pKMF016 | + | − | 4.98 ± 0.64 | 1.43 ± 0.40 | 0.48 ± 0.06 |
PAO1/pKMF014 | + | + | 14.46 ± 2.17 | 2.58 ± 0.43 | 2.47 ± 0.39 |
KG2225/pKMF016 | − | − | 4.26 ± 0.40 | 1.50 ± 0.32 | 0.58 ± 0.10 |
KG2225/pKMF014 | − | + | 15.02 ± 1.45 | 2.52 ± 0.17 | 2.45 ± 0.02 |
OCR1/pKMF016 | ++ | − | 4.50 ± 0.64 | 1.54 ± 0.35 | 0.55 ± 0.08 |
OCR1/pKMF014 | ++ | + | 16.38 ± 2.17 | 2.56 ± 0.31 | 2.42 ± 0.14 |
As described in Results, for the permeability test, P. aeruginosa cells carrying the respective plasmids grown to exponential phase were further incubated for 2 h in L broth containing 1 mM IPTG.
+, wild-type level; −, nonexpression; ++, overexpression.
The results are presented as the mean ± standard deviation of three experiments.