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Representative melting curve examples of various proteins (5 µM) and 5 × SYPRO Orange in 50 mM HEPES buffer at pH 7.5. (A) SARS-CoV2 main protease (Mpro), (B) S. aureus sortase A (srtA), (C) T. cruzi cathepsin L (cruzain), (D) bovine thrombin, (E) hen egg-white lysozyme, (F) bovine serum albumin (BSA), (G) human calpain I, (H) Zika virus (ZIKV) NS2B/NS3 protease.
