Table 1.

Overview of various protein melting points and S/N-ratios as recorded with the openDSF instrument in comparison to respective literature values. DSF reaction mixtures contained 5 µM protein and 5 × SYPRO Orange in 50 mM HEPES buffer at pH 7.5.

Protein	Melting point [°C]	N (replicates)	Lit. melting point [°C]	S/N [dB]
Mpro	57.09 ± 0.08	12	55.74[31]	27.1 ± 0.32
SrtA	49.84 ± 0.13	16	50.50[32]	31.1 ± 0.32
Cruzain	64.90 ± 0.14	8	66.40[33]	30.5 ± 0.29
Thrombin	52.10 ± 0.08	4	58.30[30]	28.8 ± 0.29
Lysozyme	67.58 ± 0.10	4	71.90[29]	27.3 ± 0.27
BSA	58.72 ± 0.13	4	56.00[3]	21.6 ± 0.53
Calpain I	44.60 ± 0.11	4	47.00[34]	20.5 ± 0.16
NS2B/NS3	50.01 ± 0.07	4	49.00[35]	27.9 ± 0.26