TABLE 2.

Amino acid substitutions^a in QRDR of GyrA in B. fragilis group strains compared to ATCC strains, and MIC of four quinolones

Species	Type strain or no. of isolates	Amino acid at position:														MIC (μg/ml)
		30	40	49	53	61	68	80	82	83	86	88	94	100	101	Ciprofloxacin	Levofloxacin	Moxifloxacin	Clinafloxacin
B. fragilis	ATCC 25285	S	F	Y	E	P	I	G	S	S	F	M	E	P	L	8.0	0.5	0.125	0.125
	3	S	F	Y	E	P	I	G	S	S	F	M	E	P	L	2–>256	1–2	0.125–0.5	0.125–0.25
	1	A	F	Y	E	A	I	G	S	S	G	L	D	P	L	4	2	1	0.25
	4	A	L	Y	E	A	I	G	L	S	G	L	D	P	L	256	128	16–32	8–32
B. distasonis	ATCC 8503	S	F	F	E	P	I	G	S	S	F	M	T	P	L	8	8	0.25	0.125
B. ovatus	ATCC 8483	S	F	Y	E	P	I	G	S	S	F	M	E	P	L	16	8	0.5	0.25
	1	S	F	Y	E	P	I	G	S	S	Y	M	E	P	L	8	4	1	0.25
	1	S	F	Y	E	P	I	G	C	S	F	M	E	P	L	16	8	2	0.5
	1	S	F	Y	E	P	I	G	F	S	F	M	E	P	L	256	256	2	2
B. thetaiotaomicron	ATCC 29741	S	F	Y	E	P	I	G	S	S	Y	M	E	P	L	16	8	0.5	0.25
	3	S	F	Y	E	P	I	G	S	S	Y	M	E	P	L	8–64	2–8	1–4	0.25–1.0
	2	S	F	Y	E	P	I	G	S	S	F	M	E	P	L	256	128–256	32	8
B. uniformis	ATCC 8492	S	F	F	G	P	V	G	S	S	G	L	P	M	L	8	8	1	0.5
	1	S	F	F	G	P	V	G	S	S	G	L	P	M	L	32	16	2	0.5
	5	S	F	F	G	P	V	G	L	S	G	L	P	M	L	32–256	64–128	16–64	8–32
	1	A	F	Y	E	A	I	G	S	S	G	L	D	P	L	8	2	1	0.125
B. vulgatus	ATCC 8482	A	F	Y	E	A	I	G	S	S	G	L	D	P	L	16	2	1	0.125
	14	S	F	F	G	P	V	G	S	S	G	L	P	M	V	256–>256	2–>256	2–32	0.125–16
	2	S	F	F	G	P	V	D	S	S	G	L	P	M	V	256–>256	128–256	32–128	8–32
	1	S	F	F	G	P	V	G	S	S	G	L	P	M	L	256	256	32	32
	1	S	F	Y	G	P	V	G	L	S	G	L	P	M	L	>256	64	64	8
	2	S	F	F	G	P	V	G	L	S	G	L	P	M	L	>256–256	64–128	16–32	8–16
	1	S	F	F	G	P	V	G	L	S	G	L	P	M	V	256	256	32	16

^aOnly indicated when differences from type strain are abundant.