Fig. 4.

Rb1 profoundly promoted mitophagy and AMPKα phosphorylation in OGD-injured H9c2 cardiomyocytes. The expression of (A) PINK1, (B) Parkin, (C) LC3Ⅱ/LC3Ⅰ, (D) p62 and (E) p-AMPKα/AMPKα were detected using western blot analysis. Mitochondrial morphology and the colocalization of the mitochondrial with (F) PINK1, (G) Parkin and (H) LC3 were analyzed using a 63 × oil immersion lens (630 × ). Results were obtained from three independent experiments and were presented as mean ± SD. ^##P < 0.01 vs. the control group; ∗P < 0.05, ∗∗P < 0.01 vs. the OGD group.