Fig. 5.

Rb1 attenuated OGD-induced H9c2 cardiomyocytes injury through mitophagy. (A) The cell viability of H9c2 cardiomyocytes. (B) The release of LDH in culture medium of H9c2 cardiomyocytes. (C) Mitochondrial membrane potential (ΔΨm) was assessed by probe JC-1. Red fluorescence represents intact ΔΨm. Green fluorescence represents dissipation of ΔΨm. The expression of (D) PINK1, (E) Parkin, (F) LC3II/LC3I and (G) p62 were detected using western blot analysis. Mitochondrial morphology and the colocalization of the mitochondrial with (H) PINK1, (I) Parkin and (J) LC3 were analyzed using a 63 × oil immersion lens (630 × ). Results were obtained from three independent experiments and were presented as mean ± SD. ^##P < 0.01 vs. the control group; ∗P < 0.05, ∗∗P < 0.01 vs. the OGD group; ^$$P < 0.01 vs. the OGD + Rb1 group.