Rb1 effectively improved myocardial ischemia through AMPKα mediated mitophagy. (A) The cell viability of H9c2 cardiomyocytes. (B) The release of LDH in culture medium of H9c2 cardiomyocytes. (C) ΔΨm was assessed by probe JC-1. The expression of (D) PINK1, (E) Parkin, (F) LC3II/LC3I and (G) p62 were detected using western blot analysis. Mitochondrial morphology and the colocalization of the mitochondrial with (H) PINK1, (I) Parkin and (J) LC3 were analyzed using a 63 × oil immersion lens (630 × ). Results were obtained from three independent experiments and were presented as mean ± SD. #P < 0.05, ##P < 0.01 vs. the control group; ∗P < 0.05, ∗∗P < 0.01 vs. the OGD group; $$P < 0.01 vs. the OGD + Rb1 group.