Table 1.

Sequences of primer pairs used for amplification of target and reference genes.

Gene1	Primer sequence	Size		Acc (reference)
SOD	GTGACCTCGGCAATGTGACT GCAGTGTGGTCCGGTAAGAG		86	NM_205064.1
GPx	AAGAACTTCGGGCACCAGGA TGTACTGCGGGTTGGTCATC		214	NM_001277853.2
HSP70	CGTCAGTGCTGTGGACAAGAGTA CCTATCTCTGTTGGCTTCATCCT		145	NM_001006685
HSP90	GATCCCCAGACACATGCCAA CTCGGTAACTGCAGGACTGG		105	NM_001109785
TLR2	CGGTCATCTCAGCTACACCA GCATCGCATGAAAGACAGGC		150	NM_204278
TLR4	CCACACACCTGCCTACATGAA GGATGGCAAGAGGACATATCAAA		63	NM_001030693
IL-1β	CCCGCCTTCCGCTACA CACGAAGCACTTCTGGTTGATG		66	NM_204524.1
IL-4	GCTCTCAGTGCCGCTGATG GAAACCTCTCCCTGGATGTCAT		60	NM_001007079.1
IL-10	CGCTGTCACCGCTTCTTCA CGTCTCCTTGATCTGCTTGATG		63	NM_001004414.2
IL-17A	AGCTGGACCACAGCGTCAAC GGCGGAGGACGAGGATCT		57	NM_204460.1
IL-22	CTTCTCACCAGCCCCCTACCTCCA GCTGACCGATGAGTCTGTTGTCAGTG		175	NM_001199614.1
TNFα	CCCATCCCTGGTCCGTAAC ATACGAAGTAAAGGCCGTCCC		77	MF000729.1
iNOS	CCTGTACTGAAGGTGGCTATTGG AGGCCTGTGAGAGTGTGCAA		66	NM_204961.1
IFNγ	AGTCAAAGCCGCACATCAAAC TTCACCTTCTTCACGCCATCA		82	NM_205149.1
Occludin	CCGTAACCCCGAGTTGGAT ATTGAGGCGGTCGTTGATG		214	NM_205128.1
ZO-1	GGAGTACGAGCAGTCAACATAC GAGGCGCACGATCTTCATAA		101	XM_413773
mTOR	CATGTCAGGCACTGTGTCTATTCTC CTTTCGCCCTTGTTTCTTCACT		77	XM_417614.6
GAPDH	CCTAGGATACACAGAGGACCAGGTT GGTGGAGGAATGGCTGTCA		64	NM_204305

GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; GPx: Glutathione peroxidase; HSP: Heat shock protein; IL: Interleukin; iNOS: Inducible nitric oxide synthase; IFNγ: Interferon gamma; SOD: Superoxide dismutase; TNFα: Tumor necrosis factor alpha; TLR: Toll Like Receptor; mTOR: Mechanistic target of rapamycin; ZO: Zonula occluden. For each gene, the primer sequence for forward (F) and reverse (R) (5ʹ-3ʹ) primers, the amplicon size (bp) and the NCBI Accession number (Acc) used for the primer design are listed.