Table 3.

Effect of dietary supplementation of Vit E/Se on relative mRNA abundance of oxidative stress, immunity, and gut integrity related genes in the jejunum of heat-stressed broiler chickens on d 28.¹

	Treatments2				Diet		Ambient temperature			Statistics3
	Control		Vit E/Se							P-value
Item	TN	HS	TN	HS	Control	Vit E/Se	TN	HS	RMSE	Diet	AT	D × AT
HSP70	1.13c	13.31a	1.34c	4.31b	7.22	2.82	1.23	8.81	3.82	<0.001	<0.001	<0.001
HSP90	1.11c	5.42a	1.36c	2.66b	3.26	2.01	1.23	4.04	1.43	0.002	<0.001	<0.001
TLR2	1.09	0.80	1.20	0.87	0.94	1.04	1.14	0.84	0.49	0.453	0.016	0.848
TLR4	1.14	1.15	1.39	1.17	1.14	1.28	1.26	1.16	0.66	0.414	0.529	0.472
IL-1β	1.14	1.03	1.20	1.00	1.09	1.10	1.17	1.02	0.60	0.930	0.306	0.749
IL-4	1.39	3.57	1.10	2.56	2.48	1.83	1.25	3.06	1.18	0.032	<0.001	0.228
IL-10	1.34	1.92	1.78	2.14	1.63	1.96	1.56	2.03	1.34	0.337	0.174	0.741
IL-17A	1.11	0.83	1.60	1.33	0.97	1.47	1.36	1.09	0.79	0.017	0.186	0.974
IL-22	1.28	3.92	1.62	4.45	2.60	3.03	1.45	4.18	2.07	0.447	<0.001	0.860
TNFα	1.27	0.82	1.48	0.73	1.05	1.10	1.37	0.77	0.83	0.759	0.002	0.406
iNOS	1.23	1.91	1.48	1.59	1.57	1.54	1.36	1.75	0.92	0.872	0.090	0.217
IFNγ	1.23	1.88	1.14	1.64	1.55	1.39	1.18	1.76	0.85	0.435	0.009	0.719
Occludin	1.05	0.85	1.11	0.88	0.95	1.00	1.08	0.87	0.33	0.579	0.015	0.843
ZO-1	1.32	2.40	1.61	2.42	1.86	2.01	1.46	2.41	0.92	0.500	<0.001	0.557

^a-cMeans with different superscripts in the same row are significantly different (P < 0.05).

¹Data represent mean values of 16 replicates per treatment.

²Control: birds fed a basal diet; Vit E/Se: birds fed a basal diet supplemented with 250 mg/kg vitamin E and 1 mg/kg selenium; TN: thermoneutral; HS: heat stress

³AT: Ambient Temperature; D: Diet.