. 2022 Mar 15;101(6):101858. doi: 10.1016/j.psj.2022.101858

Table 4.

Effect of dietary supplementation of Vit E/Se on relative mRNA abundance of oxidative stress and immunity related genes in the liver of heat-stressed broiler chickens on d 28.¹

	Treatments²				Diet		Ambient Temperature			Statistics³
	Control		Vit E/Se		Diet		Ambient Temperature			P-value
Item	TN	HS	TN	HS	Control	Vit E/Se	TN	HS	RMSE	Diet	AT	D × AT
HSP70	1.09	8.28	1.15	6.14	4.68	3.64	1.12	7.21	5.33	0.463	<0.001	0.436
HSP90	1.16^a	4.86^a	1.25^b	2.00^b	3.01	1.62	1.20	3.43	2.42	0.026	0.001	0.018
SOD	1.05	1.31	1.01	1.30	1.18	1.15	1.03	1.30	0.42	0.814	0.013	0.864
GPx	1.10	2.53	0.83	1.64	1.82	1.22	0.97	2.10	1.04	0.032	<0.001	0.246
mTOR	1.05	0.73	1.07	0.79	0.89	0.93	1.06	0.76	0.33	0.665	0.001	0.818
TLR2	1.08	1.09	0.87	1.07	1.09	0.97	0.98	1.08	0.45	0.293	0.363	0.418
TLR4	1.17	2.31	1.30	2.08	1.74	1.69	1.24	2.19	0.80	0.792	<0.001	0.361
IL-1β	1.12	1.01	1.16	1.10	1.06	1.13	1.14	1.06	0.53	0.611	0.553	0.850
IL-4	1.13	1.02	1.38	1.01	1.07	1.19	1.25	1.02	0.54	0.386	0.090	0.341
IL-10	1.14	0.89	0.96	0.80	1.01	0.88	1.04	0.84	0.57	0.355	0.164	0.757
TNFα	1.11	0.66	1.01	0.63	0.87	0.82	1.05	0.64	0.44	0.579	<0.001	0.748
iNOS	1.15	1.31	1.40	1.19	1.23	1.30	1.28	1.25	0.78	0.728	0.904	0.336
IFNγ	1.14	0.82	1.29	0.76	0.97	1.03	1.21	0.79	0.69	0.747	0.007	0.495

^a,b

Means with different superscripts in the same row are significantly different (P < 0.05).

Data represent mean values of 16 replicates per treatment.

Control: birds fed a basal diet; Vit E/Se: birds fed a basal diet supplemented with 250 mg/kg vitamin E and 1 mg/kg selenium; TN: thermoneutral; HS: heat stress

AT: Ambient Temperature; D: Diet.