Table 5.

Effect of dietary supplementation of Vit E/Se on relative mRNA abundance of oxidative stress, immunity, and gut integrity related genes in the jejunum of heat-stressed broiler chickens on d 35.¹

	Treatments2				Diet		Ambient Temperature			Statistics3
	Control		Vit E/Se							P-value
Item	TN	HS	TN	HS	Control	Vit E/Se	TN	HS	RMSE	Diet	AT	D × AT
HSP70	1.05	8.08	1.03	6.54	4.20	3.28	1.04	7.38	4.06	0.482	<0.001	0.494
HSP90	1.09	4.52	1.10	3.86	2.75	2.39	1.10	4.20	1.69	0.454	<0.001	0.451
TLR2	1.05	1.88	0.89	1.72	1.46	1.31	0.97	1.80	0.98	0.518	0.001	0.983
TLR4	1.07	0.93	0.84	1.20	1.00	1.02	0.96	1.07	0.53	0.871	0.416	0.064
IL-1β	1.09	1.23	0.88	1.08	1.16	0.98	0.99	1.15	0.53	0.187	0.214	0.824
IL-4	1.08	2.02	0.96	1.75	1.55	1.35	1.02	1.88	1.13	0.488	0.003	0.780
IL-10	1.14	1.68	0.74	1.42	1.41	1.08	0.94	1.55	0.76	0.087	0.002	0.737
IL-17A	1.12	0.47	1.33	0.42	0.79	0.87	1.22	0.44	0.55	0.564	<0.001	0.346
IL-22	1.07	2.17	0.88	1.47	1.62	1.18	0.98	1.82	1.09	0.121	0.004	0.355
TNFα	1.04	1.09	0.95	1.09	1.06	1.02	1.00	1.09	0.46	0.709	0.420	0.701
iNOS	1.05	1.15	0.90	1.15	1.10	1.02	0.97	1.15	0.48	0.500	0.141	0.537
IFNγ	1.13	0.72	0.84	0.53	0.93	0.68	0.98	0.63	0.49	0.052	0.005	0.696
Occludin	1.06	2.16	0.96	2.41	1.61	1.68	1.01	2.28	0.91	0.753	<0.001	0.450
ZO-1	1.06	2.10	0.94	2.23	1.58	1.58	1.00	2.17	0.86	0.976	<0.001	0.566

¹Data represent mean values of 16 replicates per treatment.

²Control: birds fed a basal diet; Vit E/Se: birds fed a basal diet supplemented with 250 mg/kg vitamin E and 1 mg/kg selenium; TN: thermoneutral; HS: heat stress

³AT: Ambient Temperature; D: Diet.